introduction: widespread medical usage of botulinum toxins has increased the need for the precise analysis of its biological activity. the aim of this study was production and crystallization of the botulinum type a toxins. methods: in this study, clostridium botulinum was grown and inoculated into the tripticase soya broth culture media, under anaerobic condition and toxin production was monit...

the aromatic plants are rich in essential oils characterized by a notable antimicrobial activity; for this reason, these substances can be used to delay or inhibit the growth of pathogenic or spoilage microorganisms. in this study, log probability percentage (log p%) of growth of the clostridium botulinum type a spores in brain heart infusion (bhi) broth as affected by various levels of zataria...

the aim of the study was the analysis of lithuanian honeys for the presence of the spores of clostridium botulinum. the analyses were performed on multifloral, honeydew, nectar-honeydew, rape, buckwheat, heather and linden honey bought in direct sale. prior to culture on solid media the samples were prepared by supernatant filtration method and cultured in parallel in cooked meat medium (cmm) a...

The largest reported outbreak of type C botulism in fur production animals is described. Epidemiological investigation of 117 out of 157 (response rate, 74.5%) farms revealed that 44,130 animals died or were euthanized, while 8,033 animals with milder symptoms recovered. The overall death rate in all animals at risk was 21.7%. The death rates were significantly higher in blue and shadow foxes (...

Fluorescent-antibody reagents were prepared against vegetative cells of representative strains of each physiological group and toxin type of Clostridium botulinum known to have caused botulism in humans. A fluorescent-antibody reagent was also prepared for C. botulinum type G, which has been isolated from autopsy specimens but which has not clearly been implicated in botulism. These fluorescent...

The genus Clostridium comprises a heterogeneous group of organisms for which the phylogeny and evolutionary relationships are poorly understood. The elucidation of these evolutionary relationships necessitates the use of experimental methods that can distinguish Clostridium lineages that are time and cost effective, and can be accurately and reproducibly employed in different laboratories. Mult...

The chemical forms of Clostridium botulinum 62A and 213B were prepared, and their heat resistances were determined in several heating media, including some low-acid foods. The heat resistance of C. botulinum spores can be manipulated up and down by changing chemical forms between the resistant calcium form and the sensitive hydrogen form. The resistant chemical form of type B spores has about t...